Epigenomics

Whole Genome Bisulfite Sequencing (WGBS) is a bisulfite sequencing method that enables the detection of DNA methylation at the whole-genome level. This encompasses the analysis of methylation at CpG sites and less common non-CpG sites, including CNG. The detection and quantification of methylation are of critical importance for the understanding of gene expression and other processes that are subjected to epigenetic regulation.

Reduced representation bisulfite sequencing (RRBS) is an efficient and high-throughput technique for analysing the genome-wide methylation profiles on a single nucleotide level. It combines restriction enzymes and bisulfite sequencing to enrich for areas of the genome with a high CpG content. Given its cost-effective and productive nature, RRBS is employed extensively across a range of fields.

ATAC-seq (Assay for Transposase Accessible Chromatin using sequencing) is a high-throughput sequencing technology that uses the transposase Tn5 to investigate chromatin accessibility. The core principle of ATAC-seq is to use the transposase Tn5 to cleave open chromatin regions and, in doing so, to incorporate sequencing adapters into the DNA at these regions. These adapter-tagged DNA fragments are then subjected to high-throughput sequencing to identify open chromatin regions in the cell nucleus. These regions are typically sites in the genome that are readily accessible to transcription factors and other regulatory proteins. Therefore, ATAC-seq is a powerful tool for studying epigenetic changes in gene expression regulation, cell differentiation, and disease

CUT&Tag (Cleavage Under Targets and Tagmentation) is an advanced epigenetics technology that enables high-sensitivity detection of chromatin protein binding sites with low input or even single-cell samples. Compared with traditional ChIP-seq, CUT&Tag offers a simpler workflow, lower background noise, and higher resolution, providing more precise insights into regulatory mechanisms.