The advent of high-throughput sequencing technology has facilitated a deeper comprehension of the interrelationship between genomic variation/gene expression differences and phenotype. However, traditional bulk RNA sequencing methods are targeted at a collection of cells, and the resulting data often fails to capture the full range of information present at the single-cell level, leading to the loss of significant insights. The microfluidic technology of the 10x Genomics platform enables the concurrent acquisition of gene expression data, TCR/BCR sequence information and chromatin openness information from a large number of single cells. This is achieved by sorting single cells, thus facilitating rapid and efficient labelling, sequencing and analysis of a large number of cells. The gene expression profiles and differences at the single-cell level are obtained, and large-scale single-cell expression maps are generated through in-depth and detailed analysis of complex cell populations. The project has a relatively short project cycle and a wide range of potential applications. It is capable of producing large-scale single-cell expression profiles.
