Circular RNAs (circRNAs) represent a novel class of abundant, stable, and ubiquitous RNA molecules. Unlike mRNAs, circRNAs lack poly (A) tails and 5’ caps, and are resistant to exonuclease degradation. circRNAs can act as microRNA (miRNA) or RNA-binding protein (RBP) sponges, sequestering miRNAs and sequestering these molecules and thereby regulating gene expression and transcriptional activity.
Applications
Investigation of competing endogenous RNA (ceRNA) networks
Identification of pathological targets and biomarkers
Study of regulatory mechanisms in innate immune responses
Analysis of peptide expression
Advantages
Two options for library construction
The rRNA- library retained both circular and linear RNA sequences, ensuring a comprehensive transcriptome analysis. The RNase R+ library was designed to retain only circular RNA, providing a more specific and accurate circRNA profile.
Extensive experience
We are proud to share that our clients have published over 160 studies on long non-coding RNA (lncRNA) and circular RNA (circRNA), including 25 high-impact publications (IF > 10).
Specialist team
Our team includes experienced professionals who can assist you with experimental design and data interpretation.
Reliable and fast turnaround time
A dedicated project manager ensures the results will be delivered within 20 business days of sample quality verification.
Sample Requirements
Note: Sample amounts are provided for reference only. For detailed information, please contact us with your customized requests.
Our Comprehensive Service
At LC Sciences, we offer a genome-wide microRNA expression profiling service utilizing a microarray detection system that was developed specifically for microRNA detection. Send us your total RNA sample and we’ll perform all the necessary functions from sample QC through data analysis. We can perform microarray analysis on a single sample to create a simple expression profile or we can hybridize two samples to the same microarray for “dual sample” analysis. This is very useful whenever comparison of two samples is needed such as wildtype vs. mutant or samples treated in two different ways.
Our “Total RNA to Data” comprehensive service includes: Sample QC, Sample Preparation and Labeling, microRNA Detection (hybridization to a µParaflo® microfluidics chip), Array Scan and Data Extraction, and Full Data Analysis.
In about 2-3 weeks you will receive: original and processed scan images, array layout and a list of sequences, raw and processed data, a list of differentially expressed transcripts (dual sample arrays only), and a summary of the results.
Bioinformatics Analysis Pipeline
DNA Sequencing
LC Sciences provides a variety of fast, comprehensive DNA sequencing solutions, including exome, whole-genome, whole-genome bisulfite, and reduced representation bisulfite sequencing.
Learn MoreMicrobial Sequencing
LC Sciences provides a fast, comprehensive microbial Sequencing solution including: 16S/18S/ITS Amplicon Sequencing, 5R 16S Amplicon Metagenomic Sequencing, Shotgun Metagenomic Sequencing.
Learn MoreRNA Sequencing
LC Sciences offers a range of rapid and comprehensive RNA sequencing solutions, including poly-A RNA, micro RNA, total RNA, circRNA, m6A RNA, and more.
Learn MoreEpigenomics
LC Sciences provides specialized epigenomics services, including Whole Genome Bisulfite Sequencing (comprehensive single-base resolution DNA methylation analysis), Reduced Representation Bisulfite Sequencing (targeted CpG-rich region study with lower cost), ATAC-seq (chromatin accessibility and regulatory element investigation), and CUT&Tag (high-resolution chromatin profiling for small/single-cell samples).
Learn MoreSingle Cell Sequencing
Learn MoreProteomics & Metabolomics
LC Sciences is focused on providing scientific researchers with more minute and precise protein expression-related services, including olink proteomics.
Learn MorePre-made Library Sequencing
Learn MoreAdditional Services
Our portfolio of additional services includes Bioinformatic Services, Oligomix, MicroRNA Profiling Microarray, DNA/RNA Aptamer Microarray, Epitope Mapping, and Kinase Profiling. These services are designed to support various aspects of molecular research and biomolecular analysis, providing comprehensive solutions tailored to your specific scientific needs.
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