Kinases are essential regulators of cell signaling pathways, transferring the terminal phosphate group from ATP to the serine, threonine, or tyrosine residues of target proteins. Using custom-synthesized, addressable peptide microarrays (PepArray™) containing specific kinase substrates on a custom-synthesized, addressable LC Sciences' peptide microarray (PepArray™), we can quantitatively measure kinase activity across a wide range of signaling networks.
Applications
Protein kinase substrate profiling
Large-scale mapping of kinase substrate specificity, detection and discovery of novel kinases, and validation of computationally predicted or other candidate kinase substrate peptides.
Quantitative kinase kinetics studies
Facilitates quantitative measurement of protein kinase kinetic activities (e.g., PKA studies)
Drug discovery and development
inhibitor screening, biomarker identification, and signaling pathway studies.
Quantitative Results
All assays use universal, highly phosphate-specific fluorescent dye staining. Well-designed negative and positive control references ensure reliable and quantifiable results. In situ–synthesized PKS peptide microarrays, using LC Sciences’ PepArray™ microfluidic technology, deliver more uniform spots and provide a more reliable readout of assay signals. Multiple replicates of each peptide sequence also contributes to data confidence.
High-Throughput Microarray Format
Traditional kinase assays focus on only a single or a few peptide or protein substrates. Profiling kinases on a peptide microarray offers the opportunity to study thousands of specific substrates (up to thousands of peptides per chip) in a single experiment. A single experiment using our protein kinase substrate (PKS) peptide microarray is equivalent to 41 experiments per 96-well plate. Contact us for available array configurations and specifications>>
Custom Microarray Content
Customize one of our standard microarrays or design your own PKS peptide microarray. We can synthesize a fully custom microarray designed specifically for your experiment. Our standard format includes 4,000 customer-defined peptide sequences per chip, but arrays with longer peptides and higher densities available. Ask us for assistance with the design of your PKS peptide microarray.
Comprehensive Service
LC Sciences provides a comprehensive kinase analysis service utilizing high-density protein kinase substrate (PKS) peptide microarrays based on LC Sciences' PepArray™ microfluidic technology for proteome-scale kinase profiling, quantitative measurement of kinase activities, and drug discovery research. Our Kinase Profiling comprehensive service includes:
Synthesis of a standard content or custom-designed protein kinase substrate (PKS) peptide microarray on a PepArray™ microfluidics chip
Pre-staining of each chip to evaluate synthesis quality and establish a baseline fluorescence signal before applying the kinase sample
Quality testing of the received kinase sample
On-chip enzymatic reactions
Selective staining of phosphate groups on phosphopeptides using a highly specific fluorescent dye
Array imaging and data extraction
Data analysis – Includes basic data processing (background subtraction, control- and reference-guided signal processing, list of detected signals, data averaging, results summarized in a report) is included with our array service. Optional in-depth analysis based on your specific application is available.