Using overlapping peptides as epitopes on a custom-synthesized, addressable LC Sciences' peptide microarray (PepArray™), we can systematically screen thousands of peptide sequences in a single experiment. Our proprietary microarray platform, integrated with advanced microfluidic technology, enables precise and quantitative measurement of binding interactions. This combination of high-throughput capability and quantitative precision allows for the rapid and efficient identification of high-affinity, high-specificity target-binding compounds.
Applications
Identification of specific substrates for known antibodies
Detection of immunodominant regions within antigens
Quantitative measurement of binding affinities and interaction curves
Pre-screening for cross-reactivity to aid in the development of specific antibodies
Comprehensive mapping of antibody cross-reactivity profiles
Quantitative Results
image Epitope mapping assays using LC Sciences’ PepArray™ technology are powerful tools for immunological studies, vaccine development, and biosensor development .png — illustrative figure for webpage display
Our microfluidic epitope mapping platform produces PepArray™ technology produce uniform peptide spots and enhanced binding kinetics, resulting in more reliable and quantitative signal readouts, delivering a more reliable readout of assay signals. Well-designed positive and negative controls, as well as multiple replicates of each peptide sequence, contribute to data confidence.
Application Example—Quantitative Measurement of Anti-HA Epitope Peptide Binding
High-Throughput Microarray Format
Epitope mapping on a peptide microarray offers the opportunity to study thousands (up to tens of thousands of peptides per chip) of specific binding sequences in a single experiment. One experiment using our 4K epitope-mapping peptide microarray is equivalent to 41 experiments performed on a 96-well plate. A 30K microarray format is also available. Available Arrays >>
Custom Microarray Content
Peptides are synthesized on-chip, not pre-synthesized and spotted. There is no up-front cost to pre-synthesize a peptide library. We can synthesize a completely custom, one-of-a-kind peptide microarray specifically for your experiment, delivering results that cannot be achieved with an tailored to your experimental needs. With the ability to program new sequence designs on the fly, you can quickly revise the microarray design and content to keep experiments moving forward based on previous results.
Comprehensive Service
This is a comprehensive epitope mapping service. Just send us your protein/antibody sample and we’ll perform all the necessary steps, from sample assay to data analysis. Our comprehensive Epitope Mapping service includes:
- Assistance with sequence designs
- Synthesis of a custom-designed epitope mapping peptide microarray on a PepArray™ microfluidics chip
- Pre-staining the chip to assess synthesis quality and to obtain a baseline signal before applying the sample
- All on-chip binding reactions
- Detection of bound samples using standard immunological techniques
- Array imaging, scanning, and data extraction
- Data analysis – Basic data analysis (background subtraction, control- and reference-signal-guided data processing, listing of detected signals, data averaging, and results summarized in a report) is included with our array service.
DNA Sequencing
LC Sciences provides a variety of fast, comprehensive DNA sequencing solutions, including exome, whole-genome, whole-genome bisulfite, and reduced representation bisulfite sequencing.
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LC Sciences provides a fast, comprehensive microbial Sequencing solution including: 16S/18S/ITS Amplicon Sequencing, 5R 16S Amplicon Metagenomic Sequencing, Shotgun Metagenomic Sequencing.
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LC Sciences offers a range of rapid and comprehensive RNA sequencing solutions, including poly-A RNA, micro RNA, total RNA, circRNA, m6A RNA, and more.
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LC Sciences provides specialized epigenomics services, including Whole Genome Bisulfite Sequencing (comprehensive single-base resolution DNA methylation analysis), Reduced Representation Bisulfite Sequencing (targeted CpG-rich region study with lower cost), ATAC-seq (chromatin accessibility and regulatory element investigation), and CUT&Tag (high-resolution chromatin profiling for small/single-cell samples).
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LC Sciences is focused on providing scientific researchers with more minute and precise protein expression-related services, including olink proteomics.
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Learn MoreAdditional Services
Our portfolio of additional services includes Bioinformatic Services, Oligomix, MicroRNA Profiling Microarray, DNA/RNA Aptamer Microarray, Epitope Mapping, and Kinase Profiling. These services are designed to support various aspects of molecular research and biomolecular analysis, providing comprehensive solutions tailored to your specific scientific needs.
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