The aptamer microarrays are applied in protein binding, drug candidate screening, and biosensor development.
Our comprehensive service includes sequence design support, synthesis of standard or custom DNA/RNA (2′-OMe) aptamer microarrays, and binding assays using client-supplied molecules, such as proteins, nucleic acids, carbohydrates, or other small molecules.
Applications
Protein-Nucleic Acid Binding
Drug Screening
Target Validation
Biosensor Screening
Biomarker Screening
Diagnostic Chip Development
Inhibitor Assays
Advantages
We provide a comprehensive service for aptamer screening and binding optimization.
Simply send us your sample—we’ll screen or optimize its binding to aptamer sequences, and provide you with the data within 2–4 weeks.
Our aptamer binding assays use single- or dual-color detection, as well as quantitative measurements of binding, image data processing, and signal data processing. Within 2–4 weeks of receiving your sample(s), we provide you with the results, representative and original array images, and raw data. We also offer additional services for in-depth binding data analysis and curve fitting of quantitative measurements.
Our standard aptamer microarray contains more than 1,000 sequences. A small number of customer-specified sequences can be added at no extra cost. Alternatively, you may choose a completely custom microarray, with thousands of customer-specified aptamer sequences synthesized in situ on an addressable high-density microchip.
LC Sciences can also assist with custom aptamer sequence design. The protein assay is performed on a µParaflo® biochip, leveraging powerful microfluidic µParaflo® technology. Microfluidic circulation enables efficient sample–aptamer contact and uniform binding across all sites.
Quality Assurance:
- Efficient blocking procedures to minimize background signals.
- Uniform binding responses across all positive-binding sequences.
- Reproducible binding on redundant sites.
Additionally:
- Each assay is conducted under optimized binding protocols and precisely controlled temperature conditions.
- Each aptamer microarray is subjected to rigorous quality control testing.
- The integrity of the received protein sample is determined via a thorough analysis process. Samples that do not pass QC are flagged and the client is promptly notified with a recommendation not to proceed with microarray assays.
Applications:
LC Sciences also offers a range of specialty genomics microarrays. Please contact us!
Our Comprehensive Service
At LC Sciences, we offer a genome-wide microRNA expression profiling service using a microarray detection system developed specifically for microRNA detection. Send us your total RNA sample, and we’ll perform all the necessary steps, from sample QC through data analysis. We can perform microarray analysis on a single sample to create an expression profile, or hybridize two samples on the same microarray for dual-sample analysis. This is especially useful when comparing two samples, such as wild-type vs. mutant, or samples treated in different ways.
Our comprehensive “Total RNA to Data” service includes: sample QC, sample preparation and labeling, microRNA detection (hybridization to a µParaflo® microfluidics chip), array scanning and data extraction, and full data analysis.
Within 2–3 weeks, you will receive: original and processed scan images, the array layout and list of sequences, raw and processed data, a list of differentially expressed transcripts (dual-sample arrays only), and a summary report of the results.
DNA Sequencing
LC Sciences provides a variety of fast, comprehensive DNA sequencing solutions, including exome, whole-genome, whole-genome bisulfite, and reduced representation bisulfite sequencing.
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LC Sciences provides a fast, comprehensive microbial Sequencing solution including: 16S/18S/ITS Amplicon Sequencing, 5R 16S Amplicon Metagenomic Sequencing, Shotgun Metagenomic Sequencing.
Learn MoreRNA Sequencing
LC Sciences offers a range of rapid and comprehensive RNA sequencing solutions, including poly-A RNA, micro RNA, total RNA, circRNA, m6A RNA, and more.
Learn MoreEpigenomics
LC Sciences provides specialized epigenomics services, including Whole Genome Bisulfite Sequencing (comprehensive single-base resolution DNA methylation analysis), Reduced Representation Bisulfite Sequencing (targeted CpG-rich region study with lower cost), ATAC-seq (chromatin accessibility and regulatory element investigation), and CUT&Tag (high-resolution chromatin profiling for small/single-cell samples).
Learn MoreSingle Cell Sequencing
Learn MoreProteomics & Metabolomics
LC Sciences is focused on providing scientific researchers with more minute and precise protein expression-related services, including olink proteomics.
Learn MorePre-made Library Sequencing
Learn MoreAdditional Services
Our portfolio of additional services includes Bioinformatic Services, Oligomix, MicroRNA Profiling Microarray, DNA/RNA Aptamer Microarray, Epitope Mapping, and Kinase Profiling. These services are designed to support various aspects of molecular research and biomolecular analysis, providing comprehensive solutions tailored to your specific scientific needs.
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