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Whole Genome Bisulfite Sequencing (WGBS) is a bisulfite sequencing method that enables the detection of DNA methylation at the whole-genome level. This encompasses the analysis of methylation at CpG sites and less common non-CpG sites, including CNG. The detection and quantification of methylation are of critical importance for the understanding of gene expression and other processes that are subjected to epigenetic regulation.
Applications
Construct genomic-scale DNA methylation profiles
Investigate for DNA methylation patterns in diseases
Explore the spatiotemporal-specific modification of genomic DNA methylation
Advantages
Unsurpassed data quality
It is our policy to provide single base resolution in our coverage area and a bisulfite conversion rate of at least 99%
Wide detection range
WGBS offers a comprehensive range of assays that facilitate the genome-wide detection of methylation sites
Professional team
We can provide assistance with the design of experiments and the interpretation of data from our team of experienced experts
Reliable and fast turnaround time
A dedicated project manager could be assigned, and the results would be provided within 30 business days of sample quality verification
Sample Requirements
Note: Sample amounts are provided for reference only. For detailed information, please contact us with your customized requests.
Our Comprehensive Service
At LC Sciences, we offer a genome-wide microRNA expression profiling service utilizing a microarray detection system that was developed specifically for microRNA detection. Send us your total RNA sample and we’ll perform all the necessary functions from sample QC through data analysis. We can perform microarray analysis on a single sample to create a simple expression profile or we can hybridize two samples to the same microarray for “dual sample” analysis. This is very useful whenever comparison of two samples is needed such as wildtype vs. mutant or samples treated in two different ways.
Our “Total RNA to Data” comprehensive service includes: Sample QC, Sample Preparation and Labeling, microRNA Detection (hybridization to a µParaflo® microfluidics chip), Array Scan and Data Extraction, and Full Data Analysis.
In about 2-3 weeks you will receive: original and processed scan images, array layout and a list of sequences, raw and processed data, a list of differentially expressed transcripts (dual sample arrays only), and a summary of the results.
Bioinformatics Analysis Pipeline
DNA Sequencing
LC Sciences provides a variety of fast, comprehensive DNA sequencing solutions, including exome, whole-genome, whole-genome bisulfite, and reduced representation bisulfite sequencing.
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LC Sciences provides a fast, comprehensive microbial Sequencing solution including: 16S/18S/ITS Amplicon Sequencing, 5R 16S Amplicon Metagenomic Sequencing, Shotgun Metagenomic Sequencing.
Learn MoreRNA Sequencing
LC Sciences offers a range of rapid and comprehensive RNA sequencing solutions, including poly-A RNA, micro RNA, total RNA, circRNA, m6A RNA, and more.
Learn MoreEpigenomics
LC Sciences provides specialized epigenomics services, including Whole Genome Bisulfite Sequencing (comprehensive single-base resolution DNA methylation analysis), Reduced Representation Bisulfite Sequencing (targeted CpG-rich region study with lower cost), ATAC-seq (chromatin accessibility and regulatory element investigation), and CUT&Tag (high-resolution chromatin profiling for small/single-cell samples).
Learn MoreSingle Cell Sequencing
Learn MoreProteomics & Metabolomics
LC Sciences is focused on providing scientific researchers with more minute and precise protein expression-related services, including olink proteomics.
Learn MorePre-made Library Sequencing
Learn MoreAdditional Services
Our portfolio of additional services includes Bioinformatic Services, Oligomix, MicroRNA Profiling Microarray, DNA/RNA Aptamer Microarray, Epitope Mapping, and Kinase Profiling. These services are designed to support various aspects of molecular research and biomolecular analysis, providing comprehensive solutions tailored to your specific scientific needs.
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