16S/18S/ITS amplicon sequencing is a widely used method for the identification and differentiation of microbial species. Short hypervariable regions of conserved genes or intergenic regions—such as the 16S region of bacteria and archaea or the 18S/ITS region of fungi—are amplified by PCR and subsequently analyzed using next-generation sequencing (NGS) technology. The resulting data can be applied to numerous areas within the field of microbial research.
Applications
Explore the interactions between gut microbes and human diseases
Investigate plant–microbe interactions within roots
Study the factors influencing environmental microbial communities
Differentiate between bacteria and archaea (16S) or fungi and other eukaryotes (ITS/18S)
Advantages
Stable and reliable data
Our data analysis employs the QIIME 2 pipeline to accurately reconstruct the composition of microbial communities.
Extensive experience
We have constructed more than 300,000 libraries and assisted clients in publishing over 500 papers, nearly 100 of which have high impact factors (IF>10).
Specialist team
Our experienced staff provide expert support for experimental design and data interpretation.
Reliable and fast turnaround time
A dedicated project manager will be assigned, and results will be delivered within 30 to 50 business days after sample quality verification.
Sample Requirements
Note: Sample amounts are provided for reference only. For detailed information, please contact us with your customized requests.
Our Comprehensive Service
At LC Sciences, we offer a genome-wide microRNA expression profiling service utilizing a microarray detection system that was developed specifically for microRNA detection. Send us your total RNA sample and we’ll perform all the necessary functions from sample QC through data analysis. We can perform microarray analysis on a single sample to create a simple expression profile, or we can hybridize two samples to the same microarray for “dual sample” analysis. This is very useful whenever a comparison of two samples is needed, such as wildtype vs. mutant, or samples treated in two different ways.
Our “Total RNA to Data” comprehensive service includes: Sample QC, Sample Preparation and Labeling, microRNA Detection (hybridization to a µParaflo® microfluidics chip), Array Scan and Data Extraction, and Full Data Analysis.
In about 2-3 weeks, you will receive: original and processed scan images, array layout and a list of sequences, raw and processed data, a list of differentially expressed transcripts (dual sample arrays only), and a summary of the results.
Bioinformatics Analysis Pipeline
DNA Sequencing
LC Sciences provides a variety of fast, comprehensive DNA sequencing solutions, including exome, whole-genome, whole-genome bisulfite, and reduced representation bisulfite sequencing.
Learn MoreMicrobial Sequencing
LC Sciences provides a fast, comprehensive microbial Sequencing solution including: 16S/18S/ITS Amplicon Sequencing, 5R 16S Amplicon Metagenomic Sequencing, Shotgun Metagenomic Sequencing.
Learn MoreRNA Sequencing
LC Sciences offers a range of rapid and comprehensive RNA sequencing solutions, including poly-A RNA, micro RNA, total RNA, circRNA, m6A RNA, and more.
Learn MoreEpigenomics
LC Sciences provides specialized epigenomics services, including Whole Genome Bisulfite Sequencing (comprehensive single-base resolution DNA methylation analysis), Reduced Representation Bisulfite Sequencing (targeted CpG-rich region study with lower cost), ATAC-seq (chromatin accessibility and regulatory element investigation), and CUT&Tag (high-resolution chromatin profiling for small/single-cell samples).
Learn MoreSingle Cell Sequencing
Learn MoreProteomics & Metabolomics
LC Sciences is focused on providing scientific researchers with more minute and precise protein expression-related services, including olink proteomics.
Learn MorePre-made Library Sequencing
Learn MoreAdditional Services
Our portfolio of additional services includes Bioinformatic Services, Oligomix, MicroRNA Profiling Microarray, DNA/RNA Aptamer Microarray, Epitope Mapping, and Kinase Profiling. These services are designed to support various aspects of molecular research and biomolecular analysis, providing comprehensive solutions tailored to your specific scientific needs.
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