Poly(A) Sequencing(mRNA-seq)

Poly(A) RNA-Seq is an advanced method for accurately measuring gene expression in a biological sample under specific conditions. It enables the identification of novel transcripts, alternative splicing and gene fusion events, providing a comprehensive view of the transcriptome.

MicroRNA Sequencing(miRNA-seq)

MicroRNAs are small non-coding RNA molecules that function as negative regulators of gene expression, controlling a wide range of cellular processes. The study of microRNAs is expanding rapidly as researchers identify new microRNAs and elucidate the significance of these small regulatory elements in a multitude of biological processes.

Long Non-coding RNA Sequencing(lncRNA-seq)

Long non-coding RNAs (lncRNAs) are RNA molecules longer than 200 nucleotides that do not encode proteins. However, the involvement of lncRNAs in a wide range of important cellular processes, coupled with their critical role in human diseases such as cancer, cardiovascular disease and neurological disorders, makes them an important area of research. lncRNA-seq is a valuable tool for gaining a complete picture of the lncRNA in a biological sample under specific conditions.

Circular RNA Sequencing(circRNA-seq)

Circular RNAs (circRNAs) represent a novel class of abundant, stable and ubiquitous RNAs. circRNAs are distinguished from mRNAs in that they lack poly (A) tails and 5’ caps, and are resistant to exonuclease treatment. circRNAs can serve as miRNA or RNA-binding protein sponges, sequestering miRNAs and preventing their interactions with target mRNAs. This has the effect of controlling transcriptional events.

m6A RNA Sequencing(m6A-seq)

There has been a notable increase in interest in RNA modifications in recent years, with a particular focus on N6-methyladenosine (m6A), the most prevalent methylation modification observed in mRNA. Methylated RNA immunoprecipitation sequencing (MeRIP-seq/m6A-seq) is a highly effective method for the detection of these and other post-transcriptional RNA modifications. Furthermore, it is capable of detecting the presence of m6A on a genome-wide scale, allowing the level of modification of each gene to be clarified for group-to-group comparisons.

Single Cell RNA Sequencing (single-cell RNA-seq or scRNA-seq)

Single-cell gene expression is a technique that allows the expression of individual cells to be studied, providing a higher resolution of cellular differences than traditional RNA-Seq. Single-cell RNA-Seq allows us to understand the function of a given cell in the context of its surrounding microenvironment.

Whole Transcriptome Sequencing(WTS)

Whole Transcriptome Sequencing (WTS), which includes RNA-Seq with rRNA removal and small RNA-Seq, is a powerful tool that provides a comprehensive view of mRNAs, lncRNAs, circRNAs, as well as microRNAs, in a biological sample under specific conditions. WTS allows for the exploration of a complex post-transcriptional regulation mechanism, known as a competitive endogenous RNA (ceRNA) network. These ceRNAs, including mRNAs, lncRNAs and circRNAs, act as molecular sponges for a miRNA through their miRNA binding sites, thereby de-repressing all target genes of the respective miRNA family.

Pre-made Library Sequencing