Microbial Sequencing
LC Sciences provides a fast, comprehensive microbial Sequencing solution including: 16S/18S/ITS Amplicon Metagenomic Sequencing, 5R 16S Amplicon Metagenomic Sequencing, Shotgun Metagenomic Sequencing
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16S/18S/ITS Amplicon Metagenomic Sequencing
16S/18S/ITS amplicon metagenomic sequencing is a prevalent method for the identification and differentiation of microbial species. Short hypervariable regions of conserved genes or intergenic regions, such as the 16S region of bacteria and archaea or the 18S/ITS region of fungi, are amplified by PCR and subsequently analysed using next-generation sequencing (NGS) technology. The resulting data can be applied to a multitude of aspects within the field of microbial research.
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Shotgun Metagenomic Sequencing
Metagenomics is the study of genetic material obtained directly from complex environmental samples or human sample types (such as soil, water or gastrointestinal tract samples). Metagenomic sequencing offers insights not only into species identification, classification and quantitation of microbes, but also into functional genes, gene differences between samples and metabolic pathways.
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Human Whole Genome Sequencing
Whole genome sequencing (WGS) provides the most comprehensive insight into our genetic code. It enables the identification of genomic variation information, including single-nucleotide polymorphisms (SNPs), insertions and deletions (InDels), structural variations (SVs), and copy number variations (CNVs) in a single, cost-effective assay.
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Human Whole Exome Sequencing
It is estimated that the human exome contains approximately 180,000 exons. It is estimated that these constitute approximately 1% of the human genome, or around 30 megabases. The objective of this approach is to identify the functional variation that is responsible for both Mendelian and common diseases, while also considering the high costs associated with whole-genome sequencing and maintaining high coverage in sequence depth. It can be proposed, therefore, that whole exome sequencing (WES) represents a less costly yet still efficacious alternative to whole genome sequencing (WGS).
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Poly(A) Sequencing(mRNA-seq)
Poly(A) RNA-Seq is an advanced method for accurately measuring gene expression in a biological sample under specific conditions. It enables the identification of novel transcripts, alternative splicing and gene fusion events, providing a comprehensive view of the transcriptome.
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