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5R 16S sequencing is a microbiota analysis approach used in 16S rRNA amplicon sequencing for metagenomic studies. This method employs multiplexed PCR amplification and sequencing of five distinct regions of the 16S rRNA gene, improving coverage and reducing interference from host DNA during bacterial 16S rDNA amplification—particularly in tissue samples. As part of an advanced 16S metagenomic workflow, it also incorporates a dedicated contaminant filtration process. Together, these features enable more comprehensive and accurate profiling of microbiota composition and function, even in samples with low microbial biomass and a high proportion of host DNA.
Applications
Reveal the role of microbes in tumors development and progression
Identify the bacterial population distributions in the degraded FFPE samples
Explore microbial-disease interactions
Elucidate the composition and functional characteristics of the microbiome in tumour tissues
5R 16S Amplicon Metagenomic Sequencing Advantages
Longer regional coverage
Sequencing encompasses five regions of the 16S rRNA gene, covering about 68% of the total gene length
Identification of additional species
The extension of 16S rRNA gene coverage enables more precise identification of additional species
High detection sensitivity
The method is capable of detecting microbes in low-biomass samples, such as those derived from tumor tissues
High sample tolerance
This method is suitable for detecting organisms in degraded samples
Please note that the sample amounts listed are for reference only. If you require any further detailed information, please contact us with your customised requests.
Our Comprehensive Service
At LC Sciences, we offer a genome-wide microRNA expression profiling service utilizing a microarray detection system that was developed specifically for microRNA detection. Send us your total RNA sample and we’ll perform all the necessary functions from sample QC through data analysis. We can perform microarray analysis on a single sample to create a simple expression profile or we can hybridize two samples to the same microarray for “dual sample” analysis. This is very useful whenever comparison of two samples is needed such as wildtype vs. mutant or samples treated in two different ways.
Our “Total RNA to Data” comprehensive service includes: Sample QC, Sample Preparation and Labeling, microRNA Detection (hybridization to a µParaflo® microfluidics chip), Array Scan and Data Extraction, and Full Data Analysis.
In about 2-3 weeks you will receive: original and processed scan images, array layout and a list of sequences, raw and processed data, a list of differentially expressed transcripts (dual sample arrays only), and a summary of the results.
Bioinformatics Analysis Pipeline
DNA Sequencing
LC Sciences provides a variety of fast, comprehensive DNA sequencing solutions, including exome, whole-genome, whole-genome bisulfite, and reduced representation bisulfite sequencing.
Learn MoreMicrobial Sequencing
LC Sciences provides a fast, comprehensive microbial Sequencing solution including: 16S/18S/ITS Amplicon Sequencing, 5R 16S Amplicon Metagenomic Sequencing, Shotgun Metagenomic Sequencing.
Learn MoreRNA Sequencing
LC Sciences offers comprehensive RNA sequencing services, including poly(A), microRNA, total RNA, circRNA, and m6A sequencing. LC Sciences RNA-seq services enable accurate and efficient transcriptomic analysis.
Learn MoreEpigenomics
LC Sciences provides specialized epigenomics services, including Whole Genome Bisulfite Sequencing (comprehensive single-base resolution DNA methylation analysis), Reduced Representation Bisulfite Sequencing (targeted CpG-rich region study with lower cost), ATAC-seq (chromatin accessibility and regulatory element investigation), and CUT&Tag (high-resolution chromatin profiling for small/single-cell samples).
Learn MoreSingle Cell Sequencing
As one of the trusted in single cell sequencing companies, our advanced single-cell sequencing services support research in cellular biology and precision medicine, including scRNA-seq, immune profiling, and single-cell ATAC-seq to reveal cellular heterogeneity and deeper biological insights.
Learn MoreProteomics & Metabolomics
LC Sciences is focused on providing scientific researchers with more minute and precise protein expression-related services, including olink proteomics, positioning itself as a reliable proteomics company dedicated to advancing high-quality proteomic research and analysis.
Learn MorePre-made Library Sequencing
Learn MoreAdditional Services
Our portfolio of additional services includes Bioinformatic Services, Oligomix, MicroRNA Profiling Microarray, DNA/RNA Aptamer Microarray, Epitope Mapping, and Kinase Profiling. These services are designed to support various aspects of molecular research and biomolecular analysis, providing comprehensive solutions tailored to your specific scientific needs.
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