Albinism, a phenomenon characterized by pigmentation deficiency on the ocular side of Japanese flounder (Paralichthys olivaceus), has caused significant damage and limited mRNA and microRNA (miRNA) information is available on fish pigmentation deficiency. In a recent study, a team led by scientists from the Qingdao National Laboratory for Marine Science and Technology employed RNA sequencing to identify the mRNA and miRNAs involved in P. olivaceusalbinism.
Through high-throughput mRNA and miRNA sequencing and analysis, a series of interesting mRNA and miRNAs involved in fish pigmentation were identified in this study. The miRNA-mRNA regulatory networks also provide a solid starting point for further elucidation of fish pigmentation deficiency.
The volcanoplot of differentially expressed genes in RNA-seq
The differentially expressed genes including 41 up-regulated genes and 194 down-regulated genes in PO_alb vs PO_con were illustrated in a volcanoplot (fold change≥2 and q-value≤0.05)