In a recent study, researchers from the Chinese Academy of Fishery Sciences built control (28 °C) and heat-treated (37.5 °C) groups, and extracted RNA from the liver tissues for high-throughput next-generation sequencing to study the miRNA and mRNA expression profiles. They identified 28 differentially expressed (DE) miRNAs and 744 DE mRNAs between the control and heat-treated groups and annotated them using the KEGG database. A total of 38 target genes were predicted for 21 of the DE miRNAs, including 64 negative miRNA–mRNA interactions. They verified 15 DE miRNA–mRNA pairs and 16 other DE mRNAs by quantitative real-time PCR. Important regulatory pathways involved in the early response of GIFT to heat stress included organism system, metabolism, and diseases.
These findings facilitate the understanding of regulatory pathways affected by acute heat stress, which will help to better prevent heat damage to GIFT.
Conservation profiles of the identified GIFT liver miRNAs with miRNAs from other fish species, including Salmo salar (ssa), Ictalurus punctatus (ipu), Fugu rubripes (fru), Tetraodon nigroviridis (tni), Zebrafish (dre), and Oryzias latipes (ola).