Zhejiang University researchers used LC Sciences’ RNA sequencing services to construct a library of female ovaries from L. sticticalis and sequence the transcriptome with RNA-Seq technology. They analyzed the gene expression profile with Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG), and Clusters of Orthologous Groups (COG) for eukaryotic complete genome analysis. The RNA-seq libraries generated over 62 640 808 clean reads, and a total of 22 628 genes were identified. Many genes related to oocyte meiosis, oocyte maturation, cell adhesion, and signal transduction were identified. Twenty-eight oocyte meiosis- and oocyte maturation-related genes were selected for quantitative real-time (qRT) PCR and revealed differentially expressed genes among ovary grades (I–IV) of females. Of the 28 genes selected, 12 were highly expressed in one of the four ovary grades. These genes have potential use as molecular markers for ovary grading serve as a resource for forecasting population outbreaks of L. sticticalis.
Clustering (top panel) and a heatmap (bottom panel) of the relative expression of 28 oocyte development-related genes of Loxostege sticticalis. The four ovary grades were clustered based on the relative expression of these 28 genes. Black color indicates no expression.