In a recent study, miRNA expression profiles were identified via high-throughput sequencing in the kidney of Carassius auratus gibelio infected or uninfected with CyHV-2. The results showed that a total number of 840 known miRNAs and 48 putative novel miRNAs were identified. Comparing the expression patterns of miRNAs in the two groups, 23 miRNAs were found to be significantly differentially expressed between the uninfected and infected groups. Further, the expressions of 23 miRNAs were validated by quantitative reverse transcription polymerase chain reaction (qRT-PCR) and the results showed that the expression patterns were basically the same as the sequencing data indicated. Prediction of targets of differentially expressed miRNAs revealed that the miRNAs participated in the regulation of multiple immune-related signaling pathways, including Chemokine signaling pathway, Apoptosis, Jak-STAT signaling pathway and MAPK signaling pathway.
Taken together, these data provide insight into the regulatory mechanisms of miRNA and highlight the function of miRNA in the regulation of the immune response during the interaction between host and virus pathogens.