A total of 107 differentially expressed miRNAs (57 upregulated and 50 downregulated) were identified in MDA‑MB‑231 cells compared with MCF‑7 cells. Five prominently dysregulated miRNAs (miR‑200c‑3p, miR‑221‑3p, miR‑222‑3p, miR‑192‑5p and miR‑146a) were further confirmed by reverse transcription‑quantitative polymerase chain reaction. In addition, gene ontology analysis and pathway enrichment analysis revealed that the dysregulated miRNAs and predicted targets were found to be involved in the mitogen‑activated protein kinase, Wnt, and transforming growth factor‑β signaling pathways, which were known to contribute to TNBC progression and metastasis. Finally, miRNA gene network analyses suggested that miR‑200c may serve as a crucial miRNA in breast cancer.
Taken together, these findings may provide a comprehensive view of the function of aberrant miRNAs involved in TNBC, and dysregulated miRNAs hold promise as potential biomarkers and therapeutic targets for patients with TNBC.
KEGG pathway enrichment analysis performed by using DAVID. The top 20 highly enriched KEGG pathways are presented. KEGG, Kyoto Encyclopedia of Genes and Genomes database; DAVID, Database for Annotation, Visualization and Integrated Discovery.