MicroRNAs are short, single-stranded non-coding RNA molecules that function as regulators of tumor progression in various cancers, including glioma. A recent study by a team from Inner Mongolia Medical University sought to investigate the biological functions of miR-9-3p in glioma progression.

The results of microRNA microarray analysis indicated that microRNA-9-3p (miR-9-3p, miR-9*) is down-regulated in high-grade (grades III and IV) gliomas compared with non-tumor tissues. These results were confirmed with real-time PCR. The miR-9-3p expression level was found to be associated with age and tumor grade. Herpud1 was regulated by miR-9-3p in glioma cells and tissues and was identified as a miR-9-3p target with luciferase reporter assays. Glioma cells transfected with miR-9-3p mimics or HERPUD1-RNAi had more apoptotic cells than them in control after induced by H2O2.

These results indicated that low expression of miR-9-3p results in a high level of Herpud1, which may protect against apoptosis in glioma.

journal.pone.0174839.g001 (1)

 

Schematic diagram of the putative miR-9-3p binding site in the UTRs of Herpud1

 

Reference
Yang L, Mu Y, Cui H, Liang Y, Su X (2017) MiR-9-3p augments apoptosis induced by H2O2 through down regulation of Herpud1 in glioma. PLoS ONE 12(4): e0174839. doi: 10.1371/journal.pone.0174839 [article]

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