Astragalus Polysaccharide (APS) is an important feed additive due to its immunomodulatory functions. Previous studies have proven that miRNAs play important roles in posttranscriptional gene regulation. Researchers from Northwest A&F University recently sought to identify differentially expressed miRNAs in testes in responses to APS dietary supplements and to find the effects of APS on breeder cock testes.

Their study measured several enzymatic activities in testes and sperm samples and used miRNA sequencing service to generate miRNA expression profiles of testes from breeder cocks fed with control diets and extra APS. Researchers found APS could increase testicular functional activities of marker enzymes. Meanwhile, there were 16 up-regulated and 17 down-regulated miRNAs in APS group, compared with the control group meeting the criteria of P-values < 0.05. Twelve differentially expressed miRNAs were validated by Mir-XTM miRNA RT-qPCR. Further GO and KEGG analyses of target genes for differentially expressed miRNAs revealed that some miRNAs may be involved in testicular nutrient metabolisms and NK cell mediated cytotoxicity pathway. Moreover, the effect of dietary APS supplements on NK cell mediated cytotoxicity pathway was also validated by RT-qPCR.

These results provide novel insight into the effect of dietary APS supplements on testicular miRNA expression profiles and enzymatic changes of breeder cocks.

MiR-16–5p mimic or negative control (miR-NTC) and luciferase reporter genes with MAP2K1, MAP2K2, SH3BP2, or IFNGR2 3′UTRs in psiCHECKTM-2 vectors were co-transfected into HEK293T cells.LC Sciences

Note: (A) The overexpression of miR-16–5p was detected at 48 h in miR-16-5p group after transfection. (B) Renilla luciferase activity was normalized to firefly luciferase. All measurements shown are the means ± SE of four independent experiments.

 

Reference
S. Wu, X. Ren, Y. Li, W. Guo, X. Lei, J. Yao, X. Yang (2017) Effect of dietary Astragalus Polysaccharide supplements on testicular miRNA expression profiles and enzymatic changes of breeder cocks Sci. Reports doi: 10.1038/srep38864 [article]

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