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The objective of a recent study by researchers from the No. 7 People’s Hospital in Zhengzhou was to investigate the molecular mechanism of atrial fibrillation (AF), as well as the negative regulatory relationship between miR-29a-3p and CACNA1C. Searching the online miRNA database (www.mirdb.org), they identified the miR-29a-3p binding sequence within the 3’-UTR of the target gene and conducted luciferase assay to verify it. Cells were transfected with miR-29a-3p and ICa,L was determined in those cells...
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TACO1
The recognition and translation of mammalian mitochondrial mRNAs are poorly understood. Therefore, to gain further insights into these processes in vivo, researchers from the University of Western Australia characterized mice with a missense mutation that caused loss of the translational activator of cytochrome oxidase subunit I (TACO1)...
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The health benefits of the plant-derived polyphenol resveratrol were established in multiple disease systems. Notably, pre-treatment with resveratrol was shown to be neuroprotective in several models of cerebral ischemia. Mechanisms of resveratrol-mediated neuroprotection have been explored in the context of canonical resveratrol targets, but epigenetic and non-coding RNA processes have not yet been evaluated...
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IW000064
A Large number of short interfering RNAs (siRNAs) are generated from plant viruses during infection, but their function, character and biogenesis are unclear. In a recent study by researchers from Zhejiang Gongshang University, the viral small RNA profile in tomato plants upon Cucumber mosaic virus (CMV) Fny strain infection were analyzed using high-throughput deep sequencing techniques...
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halo-blight-bean
A genome-wide analysis identified the set of small RNAs (sRNAs) from the agronomical important legume Phaseolus vulgaris (common bean), including novel P. vulgaris-specific microRNAs (miRNAs) potentially important for the regulation of the rhizobia-symbiotic process. Generally, novel miRNAs are difficult to identify and study because they are very lowly expressed in a tissue- or cell-specific manner. In recent work by researchers from Universidad Nacional Autónoma de México, investigators aimed to analyze sRNAs from common bean root hairs (RH), a single-cell model, induced with pure Rhizobium etli nodulation factors (NF), a unique type of signal molecule...
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Lung cancer is the most common malignancy worldwide. A recent study by researchers from The 117th Hospital of PLA aimed to identify miRNA biomarkers of lung adenocarcinoma and to investigate their molecular mechanisms. In their study, miRNA expression profiling of tumor tissues and adjacent normal tissues from 10 patients were detected using miRNA microarray service. Differentially expressed miRNAs (DEMs) were identified, and were verified using quantitative reverse transcription-PCR. Thereafter, correlations between DEM expression and clinicopathological features were determined in 49 patients...
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teleost
Within vertebrates, teleost fishes provide a rich evolutionary context for studying the mechanisms of dental divergence because of the numerous axes along which their teeth have diverged phenotypically and presumably developmentally. Using both a review of teleost in situ hybridization and de novo transcriptome sequencing in a cichlid fish, a team led by researchers from the University of Konstanz examined whether 341 gene homologs thought to play a role in developing mice teeth are expressed in the tooth-bearing jaws of teleosts. The similarities and putative differences in gene expression documented between the two most commonly used models, zebrafish and cichlids, highlight what can be learned from using a greater diversity of teleost model systems in studies of tooth development. Both types of gene expression analysis also provide substantial evidence for conservation of tooth gene expression from teleosts to mammals as well as between initial and replacement teeth...
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VariantPro
VariantPro™ is an innovative multiplex PCR-based targeted sequencing method that accomplishes target selection, library preparation and molecular tagging in a simple one-step workflow. The method incorporates novel Relay-PCR™ and Omega Primer™ technologies to produce amplicons of high uniformity & specificity (Figure 1). Designed for 100% amplicon coverage (16,569 bp) of the mitochondrion genome, the VariantPro™ […]
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brain-injury-image-600x400
Traumatic brain injury (TBI) is an important health concern for which effective treatment strategies remain elusive. Understanding the complex multi-cellular response to TBI may provide new avenues for intervention. In the context of TBI, cell–cell communication is critical. One relatively unexplored form of cell–cell communication in TBI is extracellular vesicles (EVs). These membrane-bound vesicles can carry many different types of cargo between cells. Recently, microRNAs (miRNAs) in EVs have been shown to mediate neuroinflammation and neuronal injury....
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ER stress feat
MicroRNAs (miRs) regulate gene expression to support important physiological functions. Significant evidence suggests that miRs play a crucial role in many pathological events and in the cell response to various stresses. With the aim to identify new miRs induced by perturbation of intracellular calcium homeostasis, researchers from the The University of Salerno used LC Sciences miRNA microarray service to analyze miR expression profiles of thapsigargin (TG)-treated cells.
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