Their short reads were assembled into 127,038 unitranscripts and 101,209 unigenes, with average lengths of 986 and 852 bp. In total, 10,181 and 14,924 differentially expressed genes (DEGs) were detected in the CK and IBA-treated groups, respectively. Comparison of the four consecutive developmental stages showed that 282 and 260 DEGs were shared between IBA-treated and CK, suggesting that IBA treatment increased the number of DEGs. They observed 1,721 up-regulated and 849 down-regulated genes in CI vs. II, 849 up-regulated and 836 down-regulated genes in CC vs. IC, 881 up-regulated and 631 down-regulated genes in CRP vs. IRP, and 5,626 up-regulated and 4,932 down-regulated genes in CAR vs. IAR, of which 25 up-regulated DEGs were common to four pairs, and these DEGs were significantly up-regulated at AR.
Their results suggest that substantial changes in gene expression are associated with adventitious rooting. GO functional category analysis indicated that IBA significantly up- or down-regulated processes associated with regulation of transcription, transcription of DNA dependent, integral to membrane and ATP binding during the development process. KEGG pathway enrichment indicated that glycolysis/gluconeogenesis, cysteine and methionine metabolism, photosynthesis, nucleotide sugar metabolism, and lysosome were the pathways most highly regulated by IBA. They identified a number of differentially regulated unigenes, including 12 methionine-related genes and 12 ethylene-related genes, associated with the KEGG pathway cysteine and methionine metabolism. The GO enrichment, pathway mapping, and gene expression profile analyses revealed molecular traits for root induction and initiation.
This study presents a global view of the transcriptomic profiles of tetraploid black locust cuttings in response to IBA treatment and provides new insights into the fundamental mechanisms associated with auxin-induced adventitious rooting.